Only three teams featured rookies in the second leg of the conference semifinals, and all three of those teams lost. Vancouver's Tim Parker and Seattle's Oniel Fisher both started for their respective clubs, but both sides were eliminated from the competition.
17 - Miguel Aguilar - DC United - Sub vs NYRB (Leg 2), 9 mins
13 - Tim Parker - Vancouver - Started vs Portland (Leg 2),
16 - Cristian Roldan - Seattle - Sub vs FC Dallas (Leg 2), 44 mins
40 - Oniel Fisher - Seattle - Started vs FC Dallas (Leg 2), 88 mins